Human IL-17 ELISA Kit

Product overview:

The interleukin (IL)-17 family comprises six members: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F, while IL-17A is the most studied subtype. Human IL-17A is a 15-20 kDa glycosylated polypeptide, a proinflammatory cytokine primarily produced by T helper 17 (Th17) cells, alongside γδ T cells, natural killer T cells, and innate lymphoid cells. IL-17A forms both disulfide-linked homodimers (32-38 kDa) and covalent heterodimers with IL-17F (40-45 kDa). While most secreted IL-17A exists as the IL-17A:F heterodimer, the IL-17A:A homodimer is more bioactive. IL-17 plays a critical role in immune responses against extracellular pathogens and contributes to the pathogenesis of various autoimmune and inflammatory diseases.

IL-17A has attracted significant attention in scientific research due to its regulatory role in immune and inflammatory processes. IL-17 induces neutrophil recruitment and stimulates the production of pro-inflammatory cytokines like IL-6, IL-8, TNF-α, and IL-1β. Elevated IL-17A levels are associated with several chronic inflammatory diseases in humans, including rheumatoid arthritis, asthma, acute coronary syndrome, multiple sclerosis, systemic lupus erythematosus, and allograft rejection.

 

Our ELISA kit has several benefits:

  • Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
  • High sensitivity, specificity and reproducibility.
  • Fully validated in the sample types listed below.
  • 96-well plate is breakable into 12 x 8 well strips.

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Product Specification

Protein name

IL-17

Alternate names

Interleukin-17A, IL-17A, CTLA-8; CTLA8 cytotoxic T-lymphocyte-associated serine esterase 8; IL-17A cytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8

Species reactivity

Human

Assay format

Solid-phase Sandwich ELISA (quantitative)

Sample type

Serum, Plasma, Cell culture supernatant

Sample volume

100 μL

Assay length

4.5 hrs

Analytical sensitivity

<9 pg/mL

Assay range

15.6 – 1000 pg/mL

Intra-assay CV

<4%

Inter-assay CV

<11%

Recovery (%)

Serum: 98.2
Plasma: 86.3
Cell culture media: 92.2

Detection & Instrument

Colorimetric, Microplate Reader

UniProt ID

Q16552